Journal: Journal of Neuroinflammation

Article Title: Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer’s disease

doi: 10.1186/s12974-022-02599-4

Figure Lengend Snippet: Aβ 1–42 i.c.v accelerated the amyloidogenic pathways in brain and colon. A , C – F Western blot and quantification of p-APP, PS1, BACE1, and ADAM10) in brain of each group. (B, G-J) Western blot and quantification of p-APP, PS1, BACE1, and ADAM10) in colon of each group. n = 3 per group. Data were presented as mean ± SEM. Statistics were analyzed using one-way analysis of variance (ANOVA) and followed by Fisher’s least significant difference (LSD) test. ∗ P < 0.05, **P < 0.01, ns no statistical difference

Article Snippet: The primary antibodies used in this study include ZO-1 (ThermoFisher, PA5-85256), CLDN5 (ThermoFisher, 35-2500), Synapsin1 (abcam, ab64581), PSD95 (Cell Signaling Technology, 3450), M1AChR (Santa Cruz, sc-365966), CHAT (abcam, ab178850), CHRNA7 (Abclonal, A1588), CD206 (Cell Signaling Technology, 24595), CD86 (Abclonal, A19026), NF-kb (Cell Signaling Technology, 8242), p-APP (Thr668, Cell Signaling Technology, 6986), PS1 (Cell Signaling Technology, 5643), BACE1 (Abclonal, A5266), ADAM10 (Abclonal, A10438), and β-actin (Cell Signaling Technology, 3700).

Techniques: Western Blot

Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: List of the membrane proteins suggested as potential breast cancer biomarkers.

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Membrane

Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: Western blot analysis of CD63, CD81, GPC-1, GLUT-1, and ADAM10 in the exosomes derived from the MDA and MCF cell lines. Full-length gels are shown in Figure .

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Western Blot, Derivative Assay

Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: tSNE plots of exosomes analyzed by FACS showing expression levels of ( a ) GPC-1 ( b ) GLUT-1 and ( c ) ADAM10 along with CD81 and CD63 on the surface of BC-derived exosomes.

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Expressing, Derivative Assay

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Distribution of ADAM10 status in 78 human laryngeal carcinoma tissues according to clinicopathological characteristics.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: ADAM10 expression in laryngeal carcinoma tissues. (A) Analysis of the protein expression level of ADAM10 in 5 of 20 paired laryngeal carcinoma tissues by western blotting. (B) mRNA level of ADAM10 in 20 paired laryngeal carcinoma tissues, as determined by reverse transcription-quantitative polymerase chain reaction analysis. The experiments were repeated ≥3 times. *P<0.05. T, laryngeal carcinoma tissues; N, adjacent non-tumor tissues; ADAM, a disintegrin and metalloproteinase; mRNA, messenger RNA.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Immunohistochemistry findings of ADAM10 expression in laryngeal carcinoma tissues and adjacent non-tumor tissues. (A and B) Low expression of ADAM10 in adjacent non-tumor tissues (original magnification, ×200 and ×400, respectively). (C and D) ADAM10 immunohistochemical staining in laryngeal carcinoma tissues (original magnification, ×200 and ×400, respectively). (E and F) Ki-67 immunohistochemical staining in laryngeal carcinoma tissues (original magnification, ×200 and ×400, respectively). Scale bar, 100 µm. ADAM, a disintegrin and metalloproteinase.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Immunohistochemistry, Expressing, Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Expression of ADAM10 and Ki-67 in 78 laryngeal carcinoma and 35 tumor-adjacent normal tissues.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Association between Ki-67 proliferation index and ADAM10 expression in laryngeal carcinoma. Scatter plot of Ki-67 vs. ADAM10 expression with regression line revealed a correlation between them using the Spearman's correlation coefficient (P<0.01). ADAM, a disintegrin and metalloproteinase.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Kaplan-Meier survival curves of 78 patients with laryngeal carcinoma based on ADAM10 expression status. Patients in the high-expression group had significantly poorer prognosis than those in the low-expression group (P<0.05, log-rank test). ADAM, a disintegrin and metalloproteinase.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Survival status and clinicopathological parameters in 78 human laryngeal carcinoma tissues.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Clinical significance of ADAM10 expression in laryngeal carcinoma

doi: 10.3892/ol.2016.5546

Figure Lengend Snippet: Contribution of various potential prognostic factors to survival by Cox regression analysis on 78 human laryngeal carcinoma patients.

Article Snippet: Then, primary antibodies were used for immunoblot analysis against ADAM10 (1:300; D221496-0100; Sangon Biotech, Co., Ltd.) and β-actin (1:2,000; sc-47778; Santa Cruz Biotechnology, Inc.), overnight at 4°C.

Techniques: Expressing

Journal: eLife

Article Title: Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

doi: 10.7554/eLife.99172

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-ADAM10 11G2 , , Diaclone: #857.800.000 , WB (1/1000).

Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Marker, Sequencing, Purification, Transduction, Control, Software, Staining